Date of Award

January 2019

Document Type

Thesis

Degree Name

Medical Doctor (MD)

Department

Medicine

First Advisor

John Geibel

Abstract

The standard of care for ischemic or traumatic intestinal insults involves resection of the injured intestine, often leading to further complications associated with short bowel syndrome. Despite recent advances in the field of tissue engineering, there have been limited studies on the fabrication of functional artificial intestine. We bioprinted cylindrical tubes from rat smooth muscle cells and fibroblast cells that remained patent and were able to withstand continuous and pulsatile perfusion at supraphysiological flow rates. These prints were sized into biopatches that sealed enterotomies in the murine small intestine. Histopathological examination showed progressive villi and crypt formation over the enterotomies. At 30 days, the epithelium was completely restored and the biopatch was indistinguishable from native intestine. 3D printed biopatches are thus a novel option for the surgical repair of intestinal injuries.

Comments

This thesis is restricted to Yale network users only. This thesis is permanently embargoed from public release.

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