Tissue-Engineered Vascular Grafts Allow Neovessel Formation By Recruiting Adjacent Vascular Tissue

Author

Adam Shoffner

Date of Award

January 2012

Document Type

Open Access Thesis

Degree Name

Medical Doctor (MD)

Department

Medicine

First Advisor

Christopher Breuer

Subject Area(s)

Medicine

Abstract

Hypothesis: Tissue Engineered Vascular Grafts (TEVGs) constructed by seeding cells autologous bone marrow-derived mononuclear cells onto dissolvable scaffolding aid in the recruitment of cells from either the host's bone marrow or neighboring vasculature to form the graft neotissue.

Methods: RT-PCR was performed on explanted grafts to detect presence of seeded cells. FISH for Y chromosome was performed on female mice with male bone marrow transplant along with female mice with implanted male-composite TEVG.

Results: The seeded cells on implanted TEVGs decrease precipitously from 4.37% to 0.02% from 6hr to 14 days. At 6 months post implantation composite graft TEVGs displayed a high percentage of Y chromosome-positive cells adjacent to male inferior vena cava tissue. Explanted grafts from female mice following male bone marrow transplant revealed no neotissue positive for Y chromosome.

Discussion: TEVGs derive their neotissue from adjacent vasculature following implantation that is mechanistically aided by the presence of seeded bone marrow-derived mononuclear cells.

Comments

This is an Open Access Thesis.

Recommended Citation

Shoffner, Adam, "Tissue-Engineered Vascular Grafts Allow Neovessel Formation By Recruiting Adjacent Vascular Tissue" (2012). Yale Medicine Thesis Digital Library. 1764.
https://elischolar.library.yale.edu/ymtdl/1764