Date of Award
Medical Doctor (MD)
The standard of care for ischemic or traumatic intestinal insults involves resection of the injured intestine, often leading to further complications associated with short bowel syndrome. Despite recent advances in the field of tissue engineering, there have been limited studies on the fabrication of functional artificial intestine. We bioprinted cylindrical tubes from rat smooth muscle cells and fibroblast cells that remained patent and were able to withstand continuous and pulsatile perfusion at supraphysiological flow rates. These prints were sized into biopatches that sealed enterotomies in the murine small intestine. Histopathological examination showed progressive villi and crypt formation over the enterotomies. At 30 days, the epithelium was completely restored and the biopatch was indistinguishable from native intestine. 3D printed biopatches are thus a novel option for the surgical repair of intestinal injuries.
Maina, Renee Muyoka, "Scaffold-Free Three-Dimensional Bioprints Repair Small Intestine Injuries And Integrate Into Native Intestine" (2019). Yale Medicine Thesis Digital Library. 3514.