Title

Development of Mechanically Complex DNA Nanodevices and a Diverse DNA Origami Toolkit for Cryo-Em Imaging

Date of Award

Spring 2021

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Cell Biology

First Advisor

Lin, Chenxiang

Abstract

Mechanically interlocked supramolecular assemblies are appealing building blocks for creating functional nanodevices. Here we describe the multi-step assembly of massive DNA origami rotaxanes that are capable of programmable structural switching. We validated the topology and structural integrity of these rotaxanes by analyzing the intermediate and final products of various assembly routes using electrophoresis and electron microscopy. Additionally, we explore the use of various other DNA origami nanodevices as support structures for improved cryoEM imaging of proteins of interest. Namely, we demonstrate progress toward several DNA nanodevice-based solutions to artifacts stemming from air-water interface interactions and other freezing effects.

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